1. 外采用SECC鋼板、精粉體烤漆處理,內(nèi)為耐高溫陶瓷板;
2. 發(fā)熱體,使用壽命長;
3. 進口數(shù)位溫度表,控溫,操作簡單;
4. 升降溫快,經(jīng)濟快速;
5. 超溫保護,安全;
6. 可通氣體;
7. 循環(huán)方式:熱輻射及自然對流;
8. 溫度范圍;室度+20—1000度;
9. 溫控器:PID微電腦控制;
10.計時器:溫到計時、時間到切斷加熱電源;
11.內(nèi)外箱尺寸比例大,占空間小,容量大,控制面板可自由擺設(shè)
大鼠AP核酸內(nèi)切酶-1(APE1)酶聯(lián)免疫分析(ELISA)
試劑盒使用說明書
本試劑僅供研究使用 目的:本試劑盒用于測定大鼠血清、血漿、組織勻漿及相關(guān)液體樣本中AP核酸內(nèi)切酶-1(APE1)的含量。
實驗原理:
本試劑盒應(yīng)用雙抗體夾心法測定標本中大鼠AP核酸內(nèi)切酶-1(APE1)水平。用純化的大鼠AP核酸內(nèi)切酶-1(APE1)捕獲抗體包被微孔板,制成固相抗體,往包被的微孔中依次加入大鼠AP核酸內(nèi)切酶-1(APE1),再與HRP標記的檢測抗體結(jié)合,形成抗體-抗原-酶標抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的大鼠AP核酸內(nèi)切酶-1(APE1)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(OD值),通過標準曲線計算樣品中大鼠AP核酸內(nèi)切酶-1(APE1)含量。
試劑盒組成:
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注:標準品濃度依次為:16、8、4、2、1、0 ng/mL.
樣本處理及要求:
1. 血清:室溫血液自然凝固10-20分鐘,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清,保存過程中如出現(xiàn)沉淀,應(yīng)再次離心。
2. 血漿:應(yīng)根據(jù)標本的要求選擇EDTA或檸檬酸鈉作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清,保存過程中如有沉淀形成,應(yīng)該再次離心。
3. 尿液:用無菌管收集,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清,保存過程中如有沉淀形成,應(yīng)再次離心。胸腹水、腦脊液參照實行。
4. 細胞培養(yǎng)上清:檢測分泌性的成份時,用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。檢測細胞內(nèi)的成份時,用PBS(PH7.2-7.4)稀釋細胞懸液,細胞濃度達到100萬/ml左右。通過反復(fù)凍融,以使細胞破壞并放出細胞內(nèi)成份。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。
5. 組織標本:切割標本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存?zhèn)溆。標本融化后仍然保?span>2-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標本勻漿充分。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。分裝后一份待檢測,其余冷凍備用。
6. 標本采集后盡早進行提取,提取按相關(guān)文獻進行,提取后應(yīng)盡快進行實驗。若不能馬上進行試驗,可將標本放于-20℃保存,但應(yīng)避免反復(fù)凍融.
7. 不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
操作步驟
1. 標準品的加樣:設(shè)置標準品孔和樣本孔,標準品孔各加不同濃度的標準品50μL;。
2. 加樣:分別設(shè)空白孔(空白對照孔不加樣品及酶標試劑,其余各步操作相同)、待測樣品孔。在酶標包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl(樣品最終稀釋度為5倍)。加樣將樣品加于酶標板孔底部,盡量不觸及孔壁,輕輕晃動混勻。
3. 加酶:每孔加入酶標試劑100μl,空白孔除外。
4. 溫育:用封板膜封板后置37℃溫育60分鐘。
5. 配液:將20倍濃縮洗滌液用蒸餾水20倍稀釋后備用。
6. 洗滌:小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干。
7. 顯色:每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.
8. 終止:每孔加終止液50μl,終止反應(yīng)(此時藍色立轉(zhuǎn)黃色)。
9. 測定:以空白孔調(diào)零,450nm波長依序測量各孔的吸光度(OD值)。 測定應(yīng)在加終止液后15分鐘以內(nèi)進行。
注意事項:
1. 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標包被板開封后如未用完,板條應(yīng)裝入密封袋中保存。
2. 濃洗滌液可能會有結(jié)晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結(jié)果。
3. 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準確性,以避免試驗誤差。一次加樣時間最好控制在5分鐘內(nèi),如標本數(shù)量多,推薦使用排槍加樣。
4. 請每次測定的同時做標準曲線,最好做復(fù)孔。如標本中待測物質(zhì)含量過高(樣本OD值大于標準品孔第一孔的OD值),請先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測定,計算時請最后乘以總稀釋倍數(shù)(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6. 底物請避光保存。
7. 嚴格按照說明書的操作進行,試驗結(jié)果判定必須以酶標儀讀數(shù)為準.
8. 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。
9. 本試劑不同批號組分不得混用。
10. 如與英文說明書有異,以英文說明書為準。
計算:
以標準物的濃度為橫坐標,OD值為縱坐標,
在坐標紙上繪出標準曲線,根據(jù)樣品的OD
值由標準曲線查出相應(yīng)的濃度;再乘以稀釋
倍數(shù);或用標準物的濃度與OD值計算出標
準曲線的直線回歸方程式,將樣品的OD值
代入方程式,計算出樣品濃度,再乘以稀釋
倍數(shù),即為樣品的實際濃度。
(此圖僅供參考)
試劑盒性能:
1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上。
2.批內(nèi)變異系數(shù)與批間變異系數(shù)應(yīng)分別小于10%和15% 。
檢測范圍:
0.5 ng/mL - 16 ng/mL
靈敏度:
最低檢測濃度小于0.1 ng/mL
保存條件及有效期:
1.試劑盒保存: 2-8℃。
2.有效期: 6個月
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Drug Names
Generic Name:Rat apurinic;apyrimidinic endonuclease 1 (APE1) ELISA Kit.
Purpose
This kit allows for the determination of APE1 concentrations in Rat serum, plasma, tissue homogenates and other biological fluids.
Principle of the assay
The kit assay Rat APE1 level in the sample, use Purified Rat APE1 antibody to coat microtiter plate wells, make solid-phase antibody, then add APE1 to the wells, Combined antibody which With HRP labeled, become antibody-antigen-enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
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Note: Standard concentration was followed by:
16、8、4、2、1、0 ng/mL.
Specimen requirements
1. serum- coagulation at room temperature 10-20 mins,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2. plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3. Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4. cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS(PH7.2-7.4), Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5. Tissue samples- After cutting samples, check the weight,add PBS(PH7.2-7.4), Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS(PH7.4), Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6. extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.
2.add sample:Set blank wells separately (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.add enzyme:Add HRP-Conjugate reagent 100μl to each well, except blank well.
4.Incubate: After closing plate with Closure plate membrane ,incubate for 60 min at 37℃.
5.Configurate liquid: 20-fold wash solution diluted 20-fold with distilled water and reserve.
6.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
7.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
8.Stop the reaction:Add Stop Solution 50μl to each well, Stop the reaction(the blue color change to yellow color).
9.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1. The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2. washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3. add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4. if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.(×n×5).
5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6. The substrate evade the light preservation.
7. Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8. All samples, washing buffer and each kind of reject should according to infective material process.
9. Do not mix reagents with those from other lots.
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Calculate
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Assay range
0.5 ng/mL - 16 ng/mL
Sensitivity
The minimum detectable dose is typically less than 0.1 ng/mL
Storage and validity
1.Storage: 2-8℃.
2.validity: six months.
【中文名稱】:維生素B12
【英文名稱】:Vitamin B12
【別名】鈷胺素(cobalamin,氰鈷胺素(cyanocobalamin)
【分子式】:C63H88CoN14O14P
【作用】
已知B12是幾種變位酶的輔酶,如催化Glu轉(zhuǎn)變?yōu)榧谆?/span>Asp的甲基天冬氨酸變位酶、催化甲基丙二酰CoA轉(zhuǎn)變?yōu)殓牾?/span>CoA的的甲基丙二酰CoA變位酶。B12輔酶也參與甲基及其他一碳單位的轉(zhuǎn)移反應(yīng)。
維生素B12又叫鈷胺素,自然界中的維生素B12都是微生物合成的,高等動植物不能制造維生素B12。維生素B12是需要一種腸道分泌物(內(nèi)源因子)幫助才能被吸收的惟一的一種維生素。有的人由于腸胃異常,缺乏這種內(nèi)源因子,即使膳食中來源充足也會患惡性貧血。植物性食物中基本上沒有維生素B12。它在腸道內(nèi)停留時間長,大約需要三小時(大多數(shù)水溶性維生素只需要幾秒鐘)才能被吸收。維生素B12的主要生理功能是參與制造骨髓紅細胞,防止惡性貧血;防止大腦神經(jīng)受到破壞。
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GPD 系列流量計適用于黏度從5 到25,000 mm²/s 的各種介質(zhì)的精確測量。如果介質(zhì)的流動性很好,可以選用GPD01/1 及02,球形軸可以應(yīng)用于5mm2/s以下的介質(zhì)。齒輪流量計屬于正排量表的一種,設(shè)計結(jié)構(gòu)及原理類似于齒輪泵,介質(zhì)推動兩個齒輪轉(zhuǎn)子不停的旋轉(zhuǎn),介質(zhì)被迫從齒輪與外殼間的封閉測量腔中通過。因為齒輪沒有連帶其他的負載,所以能量損耗很小。齒輪的轉(zhuǎn)速與流速成正比, 轉(zhuǎn)速可以通過外殼完好的被檢測出來而不用與測量介質(zhì)接觸。流量信號可以用我們的VTM 表頭或其他電子設(shè)備顯示;工廠會根據(jù)客戶的操作黏度來進行標定每一臺流量計以確定真確的K系數(shù),測量的正確性! VTM 是可編程的現(xiàn)場顯示儀表,內(nèi)部帶頻率接收及放大器, 流速可以在LCD上顯示8位。10點線性調(diào)整功能可以優(yōu)化流量計的測量精度。脈沖輸出功能可以提供一個與流量成比例的頻率信號或與程序設(shè)定一致的當量脈沖信號。對于電氣連接,可以選擇6芯的插座或6個接線端子的接線盒。 過程連接:Female for,Ermeto-fittings GE 6-PSM,GE 14-PSM or GE 25-PSM, bores for SAE flanges 1¼ 操作壓力: 小尺寸可以到690 bar,大尺寸可以到400 bar; 操作溫度范圍: +180 °C; 流量范圍0.005 -- 1000 LPM; 黏度范圍5---25,000 mm²/s. 材料:外殼SS DIN 1.4305/AISI 303 或1.4571/AISI 316 Ti 齒輪SS DIN 1.4122/AISI 303 或1.4460/AISI 329 軸承,襯套炭化鎢, 球形軸承密封O-rings: viton, teflon, NBR or EPDM for brake fluid 線性±0.5% @ 1:20 黏度范圍在15 -50 mm²/s. ±0.25% of value for viscosities 50 to 25,000 mm²/s. 重量: 400 to 4000 g ALGPD Series
型號 流量范圍(參考動力粘度為1CP
XXX l/min 01 0.005 to 1 01/1 0.005 to 2 01/2 0.02 to 3 02/1 0.05 to 2 02 0.1 to 7 03 0.5 to 25 04 0.5 to 70 05 5 to 15006/1 5 to 25006 20 to 50007 50 to 1000
QL-1705 | 方型感應(yīng)距離5mm接近開關(guān) |
QL-1805 | 方型感應(yīng)距離5mm接近開關(guān) |
QL-1808 | 方型感應(yīng)距離8mm接近開關(guān) |
TS-1202 | 方型感應(yīng)距離2mm接近開關(guān) |
TS-1204 | 圓型感應(yīng)距離4mm接近開關(guān) |
TS-0801 | 圓型感應(yīng)距離1mm接近開關(guān) |
TS-0802 | 圓型感應(yīng)距離2mm接近開關(guān) |
TS-1805 | 圓型感應(yīng)距離5mm接近開關(guān) |
TS-3010 | 圓型感應(yīng)距離10mm接近開關(guān) |
TS-3015 | 圓型感應(yīng)距離15mm接近開關(guān) |
功能說明 :輸出方式:NA -NPN 常開型NB -NPN 常閉型PA -PNP 常開型PB -PNP 常閉型 外徑尺寸:18*18mm 最大感測距離:5mm ±10%安全感測範圍:0 ~4mm標準感測物體:17 ×17 ×1mm磁滯距離:15% or less of operation distance電源電壓:10-30VDC消耗電流:10mA or less輸出電流:Maximun sink current 150Ma殘留電壓:1.5V or less (at 150mA sink current)反應(yīng)頻率:600HZ外殼材質(zhì):ABS電線長度:標準 - 2米
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型號:IXS1212
輸出電壓范圍:30-120kV
輸入電壓范圍:24VDC±10%
輸出電流范圍:0.2-1.0mA
輸出功率:120W
角度:錐形:35°
焦點尺寸:0.5mm
尺寸:245*80*184mm
重量:約5.9kg
通訊方式:RS232(以太網(wǎng),無線網(wǎng))
特點:油冷、重量輕、穩(wěn)定性高、可靠性好。
典型應(yīng)用:工業(yè)無損、安檢等。 Model:IXS1212 Portable
Output kV:30-120kV
Input Line Range:24VDC±10%
Output mA:0.2-1.0mA
Battery Power:120W continuous 14min,pulsing:21min,15sec on /15sec off
Battery Charging time:2 hours from low line(21V)
X-ray Beam: Cone Beam 35°
Focal Spot Size:0.5mm as per IEX60336
Dimension: 245*80*184mm
Weight: 5.9kg(Generator only)
Communication: RS232 (Wifi,Ethernet)
Cooling: Air cooled
Features: Oil, Compact, Light Weight, High Precision output