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SV壓力傳感器,稱重傳感器（load cell）

我公司（svloadcell@hotmail.com）主要經(jīng)營壓力傳感器（load cell）、稱重傳感器,測力傳感器,拉力傳感器,張力傳感器,壓力變送器（pressure sensor），稱重系統(tǒng)（weighing system）,配料稱重系統(tǒng),罐體稱重系統(tǒng),料斗稱重系統(tǒng),電子稱，汽車稱重儀，軸重儀及附件的生產(chǎn)廠家。公司的產(chǎn)品線主要分為：應(yīng)變式測力／稱重傳感器、應(yīng)變式扭矩／多分力傳感器及壓力變送器、應(yīng)變式流體壓力傳感器及變送器和傳感器用高精度電阻式應(yīng)變計等產(chǎn)品。其中有各種型式的稱重傳感器,如橋式的,剪切梁式的,單點式,柱式的,輪輻式的,板式的,S型的,平行梁式的,同時也提供OEM,可能根據(jù)客戶要求自行設(shè)計各類稱重測力傳感器.

　　近年來，又開發(fā)了各種稱重測試臺、日用衡器用傳感器及電子衡器、高精度稱重傳感器用應(yīng)變計等新產(chǎn)品系列。

電　　話： 0510-88056151

傳　　真： 0510-88056151

郵件:svloadcell@hotmail.com

郵　　編： 214215

公司網(wǎng)站： http://www.load-cell.com.cn

RHC-200ATC

用來測量人或動物的尿蛋白，血清含量, 操作方便，精確度高

CC-118A透明槽加熱型恒溫水浴

CC-118A透明槽加熱型恒溫水浴可以方便觀察浴槽中的變化。工作溫度為100℃，接泵接口可以用于外循環(huán)，溫度穩(wěn)定性符合DIN12876標準。 CC-118A透明槽加熱型恒溫水浴技術(shù)指標內(nèi)槽體積：18L；加熱功率：2KW；溫度范圍：室溫~100℃ 開口尺寸（mm）：302＊325 外形尺寸（mm）：333＊520＊335

訂貨號：2001.0005.04

BD80/120W廣州博勵德國凱馳代理大型全自動KARCHER洗地機

產(chǎn)地/品牌︰德國凱馳Karcher

德國凱馳（廣州博勵）BD80/120W BP大型手推式自走型自動洗地吸干機

產(chǎn)品介紹：KARCHER德國凱馳（廣州博勵）BD80/120W憑借各120升凈/污水箱的超大水箱容量、的工作視野范圍、強勁耐用的技術(shù)，凱馳該款超高性能洗地吸干機，成為當今全球大型手推式地面洗地機的新寵，是您必須在行走洗地條件下的最理想選擇！

技術(shù)參數(shù)：德國凱馳Karcher（廣州博勵）全自動洗地機吸干機
洗地寬度毫米	800
吸水寬度毫米	990
凈/污水箱升	120/120
洗地效率平米/小時	3200
刷子轉(zhuǎn)速轉(zhuǎn)/分	200
刷子觸地壓力（可調(diào)）	36 克/平方厘米
電機功率瓦	1660
重量公斤	170

產(chǎn)品描述與應(yīng)用:KARCHER德國凱馳（廣州博勵）BD80/120W超高性能洗地吸干機，能夠滿足長時間、高負荷工作要求，其對向轉(zhuǎn)動的盤刷，最大化節(jié)約用水，延長您的工作時間。

應(yīng)用領(lǐng)域：可廣泛使用于工廠、倉庫、物流中心等地面的清潔與維護等…

廣州市博勵建材有限公司是德國凱馳KARCHER授權(quán)中國代理與凱馳KARCHER戰(zhàn)略級銷售中心,廣州博勵主營凱馳KARCHER全線產(chǎn)品經(jīng)銷批發(fā)與零售,凱馳清潔設(shè)備包括: KARCHER高壓清洗機,KARCHER高壓水槍, KARCHER洗地機,KARCHER掃地機,KARCHER清掃車,KARCHER吸塵器,KARCHER吸塵吸水機,KARCHER刷地機單擦機,KARCHER拋光打蠟晶面機，KARCHER地毯清洗機, KARCHER蒸汽清洗機,KARCHER零部件工業(yè)清洗機,KARCHER工業(yè)防爆吸塵器等德國凱馳KARCHER清潔設(shè)備全系列產(chǎn)品。

廣州博勵：德國KARCHER授權(quán)華南凱馳銷售中心及凱馳維修服務(wù)中心！

KARCHER凱馳原裝清潔設(shè)備及KARCHER原裝凱馳配件的！

Karcher德國凱馳廣州戰(zhàn)略級合作代理-廣州市博勵建材有限公司

該公司產(chǎn)品分類： Karcher德國凱馳掃地機 Karcher德國凱馳清潔設(shè)備配件 Karcher德國凱馳拋光打蠟單擦機 Karcher德國凱馳工業(yè)吸塵器 Karcher德國凱馳吸塵吸水機 Karcher德國凱馳真空吸塵器 Karcher德國凱馳駕駛式洗地車 Karcher德國凱馳手推式洗地吸干機 Karcher德國凱馳冷熱水高壓水槍 Karcher德國凱馳冷水高壓清洗機 Karcher德國凱馳吸塵機 Karcher德國凱馳洗地機洗地車 Karcher德國凱馳高壓清洗機

Inspector Alert α、β、γ和X射線檢測儀

儀器介紹

◆采用一只蓋革-彌勒計數(shù)管來測定α、β、γ和X射線輻射 ◆“安全第一”（Safety First）的校準功能能夠避免校準人員的輻射接觸 ◆檢測儀符合歐洲CE認證要求

主要特點

◆內(nèi)置鹵素淬滅劑GM探測器，對α、β射線源的靈敏度很高 ◆四位液晶顯示，可選擇mR/hr、CPM、mSv/hr、CPS或Total/Timer等單位 ◆總計數(shù)/定時器功能對輕微污染進行定時的精確檢測，定時時間可選擇1分鐘－－ 24小時

技術(shù)參數(shù)

◆測量范圍：mR/hr(毫倫/小時)：0.001—110.0，CPM（每分鐘計數(shù)）：0—300,000 μSv/hr(微希伏/小時)：0.01—1,100，CPS（每秒鐘計數(shù)）：0—5,000，總計數(shù)： 1—9,999,000 ◆效率：Sr-90(546kev,2.3MeV βmax)約75% C-14(156kev βmax)約11% Bi-210(1.2MeV βmax)約64% Am-241(5.5MeV α)約36% ◆靈敏度：3500CPM/ mR/hr（對于Cs-137） ◆精度：±15% ◆溫度范圍：-10℃－－-+50℃ ◆電源：1節(jié)9V堿性電池，電池壽命 200小時◆尺寸重量：150×80×30mm 350克（含電池）

應(yīng)用領(lǐng)域

◆探測和測定表面沾污◆在操作放射性核素時監(jiān)測可能存在的放射性暴露量◆調(diào)查環(huán)境污染◆測定惰性氣體及其它低能放射性核素◆建筑裝飾材料放射測定射線危害：低劑量的放射性射線輻射（天然背景輻射的變化范圍），對人體無害或風(fēng)險甚低，但達到一定劑量則會對人體有害，可引起癌癥、白內(nèi)障、不孕癥、突變、萎縮效應(yīng)、壽命減短,甚至死亡

應(yīng)用：

偵測放射性射線，以采取相應(yīng)防護措施。海關(guān)和邊境巡邏，政府執(zhí)法部門，檢疫檢驗，應(yīng)急事故處理，核電廠、銀行、政府、實驗室等部門安全巡查，醫(yī)學(xué)廢料處理，消防隊，采礦業(yè)，科學(xué)實驗，個人保護，連續(xù)監(jiān)測

參考信息（來自中國輻射防護研究院）

居民的劑量限值為每年1mSv。即0.114μSv/hr。

放射性職業(yè)人員劑量限值為每年20mSv，但任何一年不能超過50mSv。

該公司產(chǎn)品分類：核素識別儀便攜式中子測量儀便攜式β/γ/中子譜儀電離室巡測儀表面污染測量儀多功能輻射測量儀個人劑量報警儀便攜式放射性監(jiān)測儀

DDG-2090A型廠價供應(yīng) DDG-2090A型工業(yè)電導(dǎo)率儀上海博取儀器公司

DDG-2090A型工業(yè)電導(dǎo)率儀

DDG-2090A電導(dǎo)率儀是在性能的基礎(chǔ)上簡化了，從而具有了特別強的價格優(yōu)勢。清晰的顯示、簡易的操作和優(yōu)良的測試性能使其具有很高的性價比�？蓮V泛應(yīng)用于火電、化工化肥、冶金、環(huán)保、制藥、生化、食品和自來水等溶液中電導(dǎo)率值的連續(xù)監(jiān)測。

儀器特點：

微機化：采用高性能CPU芯片、高精度AD轉(zhuǎn)換技術(shù)和SMT貼票技術(shù)完成多參數(shù)測量、溫度補償、量成自動轉(zhuǎn)換，精度高，重復(fù)性好。

性：觸摸式按鍵，無開關(guān)旋鈕和電位器。

自動轉(zhuǎn)換測量頻率：避免電極極化，提高測量精度。

報警功能：報警信號隔離輸出，報警上，下限可任意設(shè)定，報警滯后撤銷。

網(wǎng)絡(luò)功能：隔離的電流輸出和RS485或RS232通訊接口

隔離電流輸出：電流對應(yīng)電導(dǎo)率的輸出上、下限可任意設(shè)定

上海博取儀器有限公司/水質(zhì)監(jiān)測儀器儀表廠家/在線電導(dǎo)率儀/工業(yè)電導(dǎo)率儀/DDG-2090A型工業(yè)電導(dǎo)率儀

技術(shù)指標：1.顯示方式：LED（數(shù)碼管）顯示、高亮度，醒目直觀，可視距離遠

2.測量范圍：（0~20000）μS/cm 溫度：0~99.9℃；

3.電極常數(shù)： 0.01～20μS/cm（配K=0.01電極）；

0.1～200μS/cm（配K=0.1電極）；

1.0～2000μS/cm（配K=1.0電極）；

10～20000μS/cm（配K=10.0電極）；

4.分辨率： 0.001μS/cm.0.1℃

5.電極常數(shù)： 0.001~15.0cm^-1間有軟件設(shè)定

6.溫補范圍：自動0~99.9℃，手動0~99.9℃，25℃折算

7.精度： ±1.0%FS，±0.3℃

8.電流隔離輸出： 4～20 mA（負載<750 Ω ）；

9.報警繼電器：AC220V 、3A；

10.通訊接口；：隔離RS485

11.電源：AC220V±22V ，50Hz±1Hz DC24V

12.防護等級：IP65；

13.工作條件：環(huán)境溫度0～60℃ 相對濕度<90%；

14.掉電保存：﹥10年；重量、尺寸：0.8 kg、96×96×110mm

15.開孔尺寸：92×92 mm；二次表安裝方式：開孔式

電極安裝方式：流通式/沉入式/法蘭式/管道式

表準配置：二次表一臺，電極一支

訂貨指南：

1. 須告知所配電極的常數(shù)

2. 電極電纜線的長度，不做說面按5m標配

3. 電極的安裝方式

該公司產(chǎn)品分類： COD分析儀氟離子檢測儀磷酸根/硅酸根分析儀鈉度計酸堿濃度計濁度儀余氯分析儀電導(dǎo)率儀 PH計溶氧儀

AN218004DWDM光譜分析儀AN218004 [1526.05—1563.86 nm] SC/PC

DWDM光譜分析儀AN218004 [1526.05—1563.86 nm] SC/PC DWDM光譜分析儀特征：·PLC 設(shè)備· DWDM（96通道）測量解決方案· 同一時間自動測量 CWDM-96通道 (波長 & 功率) · CWDM 彩色圖文掃描· 保存和調(diào)用300數(shù)據(jù)· 軟件可以自行保存數(shù)據(jù)· 自動關(guān)機DWDM光譜分析儀技術(shù)參數(shù)：

技術(shù)參數(shù)
通道數(shù)量	96	通道間隔	50GHz, 100GHz
通道頻率	196.45~191.7THz	最大輸入功率	500mW; 27dBm
帶寬	1526.05~1563.86 nm	光源接頭	SC/PC Standard
測量速度	4 Sec. (all 96ch.)	電池	鋰聚合物電池, 1800毫安時，3.7伏
測量范圍	+10~-40dBm	電池工作時長	充滿電后單次使用620分鐘
測量精度	± 1.0dB @ -40 dBm	電流消耗(Max)	0.25A
顯示屏分辨率	0.01dB	電力消耗	0.925W
顯示單位	dB, dBm,nm,THz	顯示	3.5” TFT-LCD, 16bit color, 240*320
重量	0.6 kg	尺寸	1969540 mm
溫度 (環(huán)境條件)	-20 to +55 °C (操作環(huán)境)	濕度 (最大無冷凝)	95% (操作環(huán)境)
溫度 (環(huán)境條件)	-35 to +65°C (儲存環(huán)境)	濕度 (最大無冷凝)	85% (儲存環(huán)境)

http://www.yach.com/Instrument.html 更多光學(xué)儀器儀表

該公司產(chǎn)品分類：衛(wèi)星天線慣性導(dǎo)航系統(tǒng) 天線微波射頻儀器通訊儀器儀表光通訊儀器儀表光學(xué)儀器儀表光通訊儀器儀表

GAR50-DO-2RS軸承FAG_GAR50-DO-2RS軸承參數(shù)

上海東晟軸承具有一定名氣和規(guī)模的大型進口軸承供應(yīng)商軸承型號查詢，具備多年開拓國內(nèi)外機電搜軸網(wǎng)產(chǎn)品專項營銷的廣泛渠道和較強的經(jīng)濟實力，我公司主要專業(yè)供應(yīng)GAR50-DO-2RS軸承FAG、瑞典SKF進口軸承、美國TIMKEN、德國FAG與INA等五大品牌，同時還供應(yīng)日本IKO、NTN等四個品牌，現(xiàn)貨供應(yīng)，交期短。歡迎來電咨詢GAR50-DO-2RS軸承尺寸、價格、技術(shù)支持或同型號其他品牌替換等詳細資料！如果您對FAGGAR50-DO-2RS軸承有疑問，請聯(lián)系我們獲取更多GAR50-DO-2RS軸承的最新信息 FAG軸承。FAG供應(yīng)商服務(wù)領(lǐng)域包括：冶金、電力、石化、建筑機械等，IKO軸承并在鐵路、鋼鐵、造紙、水泥、礦山等領(lǐng)域更是一枝獨秀。聯(lián)系方式：電話：傳真： NADELLA軸承GAR50-DO-2RS軸承 GAR50-DO-2RS軸承價格 GAR50-DO-2RS軸承尺寸GAR50-DO-2RS軸承報價 GAR50-DO-2RS軸承圖片NADELLA軸承【GAR50-DO-2RS軸承】尺寸參數(shù)表：軸承型號：GAR50-DO-2RS軸承品牌：FAG軸承系列：桿端軸承NADELLA導(dǎo)軌經(jīng)銷商上海東晟軸承有限公司提供FAG軸承GAR50-DO-2RS的單梁起重機、包裝設(shè)備、管道泵、化工設(shè)備配件、冷凍機、等技術(shù)參數(shù)。我公司是瑞典NSK、德國FAG和INA、美國TIMKEN軸承的進口軸承地區(qū)的經(jīng)銷商。公司秉承“質(zhì)優(yōu)、專業(yè)、高效”的經(jīng)營上海NSK軸承代理商理念，為廣大客戶提供最優(yōu)的軸承品質(zhì)、最合理的搜軸網(wǎng)價格、最滿意的服務(wù)。推薦型號：SNR7304BDB軸承 7303BDB SNR 7301BDB軸承 SNR 7300BDB SNR7302BDB SNR7240BDB軸承 7244BDB SNL528軸承 FSNL530 SNL530軸承 SNL532 FSNL532軸承 SNL312TURU SNL313TURU軸承 SNL314TURU 7321B/DT 6316-2Z軸承 24028CK30E4 W629軸承 22310RHK+H2310 7022CDB軸承 SKF軸承德國NADELLA軸承公司網(wǎng)站：http://www.bearings365.comhttp://www.zcsearch.comhttp://www.sh-bearings.comhttp://www.zhoucheng188.comhttp://www.ikont.com.cnhttp://www.shdszc.com參考資料：http://www.zhoucheng188.com/FAG/GAR50-DO-2RS.html

該公司產(chǎn)品分類：軸承

GB1800PP4MNN蘇州米頓羅GB系列機械隔膜計量泵#臺風(fēng)資訊#

上海 | 江蘇 | 浙江 | 安徽 | 福建 | 江西 | 山東 | 山西| 湖北 | 湖南 | 廣東 | 廣西 | 海南 | 重慶 | 四川 | 貴州 | 云南

蘇州米頓羅GB系列機械隔膜計量泵

GB0080 GB0180 GB0250 GB0350 GB0450	82 167 237 334 416	1.0 1.0 1.0 1.0 1.0	36 72 102 144 180
GB0500 GB0600	464 583	0.7 0.7	144 180
GB0700 GB1000 GB1200	656 946 1200	0.35 0.35 0.35	102 144 180
GB1500 GB1800	1500 1800	0.3 0.3	180 206

G系列機械隔膜計量泵特點 •可變偏心機構(gòu)調(diào)節(jié),脈動平緩 •多種材質(zhì)及泵頭結(jié)構(gòu)可選-PVC、 PVDF、316SS、高粘及漿料泵頭 •機械驅(qū)動PTFE膜片 •物料側(cè)無隔膜護盤，便于物料通過 •三種自動控制方式，適合各種控制需要 •手動/電動沖程調(diào)節(jié)可選 •沖程可調(diào)0~100%（自動/手動）

•沖程在靜態(tài)及動態(tài)條件下均可調(diào)節(jié) •大液體溫度40℃*•穩(wěn)態(tài)精度：±2%（10%~100%） •大吸程：3m水柱*•大吸入壓力：20m水柱 •可選雙隔膜泵頭結(jié)構(gòu) •建議維護周期：≤1.0MPa4000h≤0.7MPa8000h上海 | 江蘇 | 浙江 | 安徽 | 福建 | 江西 | 山東 | 山西| 湖北 | 湖南 | 廣東 | 廣西 | 海南 | 重慶 | 四川 | 貴州 | 云南

自動控制方式選擇 •電動沖程控制器接受外部控制信號，調(diào)節(jié)計量泵沖程長度，從而改變計量泵輸出流量。 G系列計量泵選擇HE型。其中GB系列可選ECC防爆型。 •變頻控制器接受外部控制信號，調(diào)節(jié)計量泵沖程速度。供電：380V/50Hz三相/220V/50Hz單相控制信號：4~20mA•電機開關(guān)控制器（VARIPULSE）：反用于GM系列計量泵，用于控制三相電機，改變沖程頻率，從而調(diào)節(jié)計量泵輸出流量。 V型-與計量泵一體式安裝，VR型-與計量泵分體式安裝

電動沖程控制器技術(shù)參數(shù)
類型	HE	ECC（GB）
供電	220V±10%,50/60Hz
控制信號	4~20mA
防護等	IP65	NEMA4
反饋信號	4~20mA	4~20mA
功耗	20VA	N/A
環(huán)境溫度	-10℃~+40℃	-40℃~+50℃
輸入阻抗	120Ω	250Ω
輸出負載	100~500Ω	N/A
執(zhí)行時間	0~100%/150S	0~100%/180S
防爆等	無	1區(qū)、IB、C和D組

潤滑油

泵型	容積	環(huán)境溫度<-5℃	環(huán)境溫度≥-5℃
GB	3L	美孚600XP68	美孚600XP220
GM	0.8L	美孚600XP68	美孚600XP220

泵頭過流部件材質(zhì)

泵頭材質(zhì)	閥體		閥座	閥球	隔膜	密封圈	進出口連接
GM0002~GM0050
PVC	PVDF		PVDF	陶瓷	PTFE	氟橡膠	PVC
PVDF	PVDF		PVDF	陶瓷	PTFE	PTFE	PVDF
316SS	316SS		316SS	316SS	PTFE	氟橡膠	316SS
GM0090~GM0500
PVC	PVC		PVC	玻璃	PTFE	氟橡膠	PVC
PVDF	PVDF		PVDF	陶瓷	PTFE	PTFE	PVDF
316SS	316SS		316SS	316SS	PTFE	氟橡膠	316SS
GB0080~GB1500
PVC	PVC		PVC	陶瓷	PTFE	氟橡膠		PVC
PVDF	PVDF		PVDF	陶瓷	PTFE	PTFE		PVDF
316SS	316SS		316SS	316SS	PTFE	氟橡膠/PTFE		316SS
GB1800
泵頭材料	閥體/襯套	閥板/升程限位板		彈簧	隔膜	密封圈	進出口連接
PVC	PVC	PVC		哈氏合金C	PTFE	氟橡膠	PVC
PVDF	PVDF	PVDF		哈氏合金C	PTFE	PTFE	PVDF
316SS	316SS	316SS		哈氏合金C	PTFE	氟橡膠	316SS

美國米頓羅GB0600機械隔膜計量泵/GB0600米頓羅加藥泵/蘇州加藥泵

美國米頓羅GB0700機械隔膜計量泵/GB0700米頓羅加藥泵/蘇州加藥泵

米頓羅GB1000機械隔膜計量泵/GB1000米頓羅加藥泵/蘇州加藥泵

美國米頓羅GB1200機械隔膜計量泵/GB1200米頓羅加藥泵/蘇州加藥泵

GB1500機械隔膜計量泵/美國米頓羅GB1500加藥泵/蘇州加藥泵

GB1800機械隔膜計量泵/美國米頓羅GB1800加藥泵/蘇州加藥泵

Ｇ系列電機驅(qū)動機械隔膜計量泵

主要性能參數(shù)

流量可達1200升/小時，壓力可達1.2MPa

無論泵是否運行，均可在0-100%范圍內(nèi)調(diào)節(jié)流量

在10%至100%范圍內(nèi)，穩(wěn)態(tài)精度為±2%

允許物料高溫度40℃

吸入提升高度可達4米水柱，允許入口壓力0.2MPa主要特性

機械驅(qū)動隔膜，物料側(cè)無隔膜護盤，便于物料通過

可變偏心機構(gòu)驅(qū)動，確保脈動平緩

鑄鋁殼體、整體重量輕

耐磨球軸承，工作更穩(wěn)定

油浴潤滑，驅(qū)動部件工作壽命更長

PVC、PP、PVDF、316SS、高粘度、漿料等多種材質(zhì)泵頭　

雙隔膜泵頭、多泵并聯(lián)等可選結(jié)構(gòu)，適合各種過程　　　　

三種不同控制方式，適合各種控制系統(tǒng)要求控制方式

電動沖程控制器：可接受外部控制信號，調(diào)節(jié)沖程長度　供電電源：220V-50Hz-單相　輸入信號：4-20mA模擬信號　輸出信號：1-5V/4-20mA模擬信號，供記錄顯示和控制系統(tǒng)使用

變頻控制器：可接受外部控制信號，調(diào)節(jié)沖程速度　供電電源：220V-50Hz-單相/380V-50Hz-3相　輸入信號：4-20mA模擬信號

馬達控制器：以"開/停"方式控制三相電機，調(diào)節(jié)輸出流量　供電電源：200-240V/50/60Hz/單相　控制方式：可接受4-20mA模擬信號、外部脈沖信號或手動調(diào)節(jié)GM/GB系列機械隔膜計量泵型號流量壓力代碼：P代碼：S代碼：T代碼：V代碼：K代碼：M系列(L/h)（Mpa)PVC316SSPVDF高粘度泵頭漿料泵頭混合物泵頭GB0080821518870081018882598986.8注意：GM0005-GM0240配0.25KW電機，可用于變頻、不放大

基本型號包括

GB0180,GB0250,GB0350,GB0450,GB0500,GB0600,GB0700,GB1000,GB1200標準配置：0.75KW電機GB150015000.3GB180018000.3

以上是米頓羅計量泵GM/GB的詳細信息，如果您對米頓羅計量泵GM/GB的價格、廠家、型號、圖片有什么疑問，請聯(lián)系我們獲取米頓羅計量泵GM/GB的新信息

該公司產(chǎn)品分類：塞瓦計量泵新道茨NEWDOSE 日本安智邁計量泵德普羅名特計量泵 SDP斯得浦計量泵美帕斯菲達計量泵意大利EMEC愛米克計量泵 TTbest精密計量泵意大利道茨計量泵意大利SEKO計量泵米頓羅計量泵力高計量泵愛力浦計量泵計量泵

CSB-EL011580RA大鼠白介素10(IL-10)ELISA KIT

Rat Interleukin 10（IL-10）

ELISA Kit

Catalog No. CSB-E04595r

（96T）

l This immunoassay kit allows for the in vitro quantitative determination of rat IL-10 concentrations in serum, plasma ａnd Tissue Homogenates.

l Expiration date six months from the date of manufacture

l FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

PRINCIPLE OF THE ASSAY

The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-10 and Avidin conjugated to Horseradish Peroxidase （HRP） is added to each microplate well ａnd incubated. Then a TMB （3,3＇,5,5＇ tetramethyl-benzidine） substrate solution is added to each well. Only those wells that contain IL-10, biotin-conjugated antibody ａnd enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution ａnd the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

DETECTION RANGE

3.12 pg/ml-200 pg/ml. The standard curve concentrations used for the ELISA’s were 200 pg/ml, 100 pg/ml, 50 pg/ml, 25 pg/ml, 12.5 pg/ml, 6.25 pg/ml, 3.12 pg/ml.

SPECIFICITY

This assay recognizes rat IL-10. No significant cross-reactivity or interference was observed.

SENSITIVITY

The minimum detectable dose of rat IL-10 is typically less than 0.78 pg/ml.

The sensitivity of this assay, or Lower Limit of Detection （LLD） was defined as the lowest protein concentration that could be differentiated from zero.

MATERIALS PROVIDED

Reagent	Quantity
Assay plate	1
Standard	2
Sample Diluent	1 x 20 ml
Biotin-antibody Diluent	1 x 10 ml
HRP-avidin Diluent	1 x 10 ml
Biotin-antibody	1 x 120μl
HRP-avidin	1 x 120μl
Wash Buffer	1 x 20 ml （25×concentrate）
TMB Substrate	1 x 10 ml
Stop Solution	1 x 10 ml

STORAGE

1. Unopened test kits should be stored at 2-8°C upon receipt ａnd the microtiter plate should be kept in a sealed bag. The test kit may be used throughout the expiration date of the kit, provided it is stored as prescribed above. Refer to the package label for the expiration date.

2. Opened test plate should be stored at 2-8°C in the aluminum foil bag with desiccants to minimize exposure to damp air. The kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

3. A microtiter plate reader with a bandwidth of 10 nm or less ａnd an optical density range of 0-3 OD or greater at 450nm wavelength is acceptable for use in absorbance measurement.

REAGENT PREPARATION

Bring all reagents to room temperature before use.

1. Wash Buffer If crystals have formed in the concentrate, warm up to room temperature ａnd mix gently until the crystals have completely dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to prepare 500 ml of Wash Buffer.

2. Standard Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 ml of Sample Diluent. This reconstitution produces a stock solution of 200 pg/ml. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making serial dilutions. The undiluted standard serves as the high standard （200 pg/ml）. The Sample Diluent serves as the zero standard （0 pg/ml）. Prepare fresh for each assay. Use within 4 hours ａnd discard after use.

3. Biotin-antibody Centrifuge the vial before opening. Dilute to the working concentration using Biotin-antibody Diluent（1:100）, respectively.

4. HRP-avidin Centrifuge the vial before opening. Dilute to the working concentration using HRP-avidin Diluent（1:100）, respectively.

Precaution: The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, ａnd clothing protection when using this material.

OTHER SUPPLIES REQUIRED

— Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

— Pipettes ａnd pipette tips.

— Deionized or distilled water.

— Squirt bottle, manifold dispenser, or automated microplate washer.

— An incubator which can provide stable incubation conditions up to 37°C±0.5°C.

SAMPLE COLLECTION AND STORAGE

l Serum Use a serum separator tube （SST）ａnd allow samples to clot for 30 minutes before centrifugation for 15 minutes at 1000 g. Remove serum ａnd assay immediately or aliquot and store samples at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.

l Plasma Collect plasma using citrate, EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 g within 30 minutes of collection. Assay immediately or aliquot ａnd store samples at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.

l Tissue Homogenates 100mg tissue was rinsed with 1X PBS, homogenized in 1 mL of 1X PBS ａnd stored overnight at -20° C. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C. The supernate was assayed ａnd removed immediately. Alternatively, aliquot ａnd store samples at -20°C or -80℃. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.

Note: Grossly hemolyzed samples are not suitable for use in this assay.

ASSAY PROCEDURE

Bring all reagents ａnd samples to room temperature before use. It is recommended that all samples, standards, ａnd controls be assayed in duplicate. All the reagents should be added directly to the liquid level in the well. The pipette should avoid contacting the inner wall of the well.

1. Add 100μl of Standard, Blank, or Sample per well. Cover with the adhesive strip. Incubate for 2 hours at 37°C.

2. Remove the liquid of each well, don’t wash.

3. Add 100μl of Biotin-antibody working solution to each well. Incubate for 1 hour at 37°C. Biotin-antibody working solution may appear cloudy. Warm up to room temperature ａnd mix gently until solution appears uniform.

4. Aspirate each well ａnd wash, repeating the process three times for a total of three washes. Wash: Fill each well with Wash Buffer （200μl）ａnd let it stand for 2 minutes, then remove the liquid by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel. Complete removal of liquid at each step is essential to good performance.

5. Add 100μl of HRP-avidin working solution to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.

6. Repeat the aspiration ａnd wash five times as step 4.

7. Add 90μl of TMB Substrate to each well. Incubate for 10-30 minutes at 37°C. Keeping the plate away from drafts ａnd other temperature fluctuations in the dark.

8. Add 50μl of Stop Solution to each well when the first four wells containing the highest concentration of standards develop obvious blue color. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.

9. Determine the optical density of each well within 30 minutes, using a microplate reader set to 450 nm.

CALCULATION OF RESULTS

Using the professional soft "Curve Exert 1.3" to make a standard curve is recommended, which can be downloaded from our web.

Average the duplicate readings for each standard, control, ａnd sample ａnd subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic （4-PL） curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis ａnd draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL-10 concentrations versus the log of the O.D. ａnd the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

LIMITATIONS OF THE PROCEDURE

— The kit should not be used beyond the expiration date on the kit label.

— Do not mix or substitute reagents with those from other lots or sources.

— It is important that the Standard Diluent selected for the standard curve be consistent with the samples being assayed.

— If samples generate values higher than the highest standard, dilute the samples with the appropriate Standard Diluent ａnd repeat the assay.

— Any variation in Standard Diluent, operator, pipetting technique, washing technique, incubation time or temperature, ａnd kit age can cause variation in binding.

— This assay is designed to eliminate interference by soluble receptors, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.

TECHNICAL HINTS

— Centrifuge vials before opening to collect contents.

— When mixing or reconstituting protein solutions, always avoid foaming.

— To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, ａnd between reagent additions. Also, use separate reservoirs for each reagent.

— When using an automated plate washer, adding a 30 second soak period following the addition of wash buffer, and/or rotating the plate 180 degrees between wash steps may improve assay precision.

— To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary.

— Substrate Solution should remain colorless or light blue until added to the plate. Keep Substrate Solution protected from light. Substrate Solution should change from colorless or light blue to gradations of blue.

— Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution. Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

該公司產(chǎn)品分類：科研試劑

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