Rat Interleukin 10(IL-10)
ELISA Kit
Catalog No. CSB-E04595r
(96T)
l This immunoassay kit allows for the in vitro quantitative determination of rat IL-10 concentrations in serum, plasma and Tissue Homogenates.
l Expiration date six months from the date of manufacture
l FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
PRINCIPLE OF THE ASSAY
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
DETECTION RANGE
3.12 pg/ml-200 pg/ml. The standard curve concentrations used for the ELISA’s were 200 pg/ml, 100 pg/ml, 50 pg/ml, 25 pg/ml, 12.5 pg/ml, 6.25 pg/ml, 3.12 pg/ml.
SPECIFICITY
This assay recognizes rat IL-10. No significant cross-reactivity or interference was observed.
SENSITIVITY
The minimum detectable dose of rat IL-10 is typically less than 0.78 pg/ml.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.
MATERIALS PROVIDED
Reagent | Quantity |
Assay plate | 1 |
Standard | 2 |
Sample Diluent | 1 x 20 ml |
Biotin-antibody Diluent | 1 x 10 ml |
HRP-avidin Diluent | 1 x 10 ml |
Biotin-antibody | 1 x 120μl |
HRP-avidin | 1 x 120μl |
Wash Buffer | 1 x 20 ml (25×concentrate) |
TMB Substrate | 1 x 10 ml |
Stop Solution | 1 x 10 ml |
STORAGE
1. Unopened test kits should be stored at 2-8°C upon receipt and the microtiter plate should be kept in a sealed bag. The test kit may be used throughout the expiration date of the kit, provided it is stored as prescribed above. Refer to the package label for the expiration date.
2. Opened test plate should be stored at 2-8°C in the aluminum foil bag with desiccants to minimize exposure to damp air. The kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
3. A microtiter plate reader with a bandwidth of 10 nm or less and an optical density range of 0-3 OD or greater at 450nm wavelength is acceptable for use in absorbance measurement.
REAGENT PREPARATION
Bring all reagents to room temperature before use.
1. Wash Buffer If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to prepare 500 ml of Wash Buffer.
2. Standard Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 ml of Sample Diluent. This reconstitution produces a stock solution of 200 pg/ml. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making serial dilutions. The undiluted standard serves as the high standard (200 pg/ml). The Sample Diluent serves as the zero standard (0 pg/ml). Prepare fresh for each assay. Use within 4 hours and discard after use.
3. Biotin-antibody Centrifuge the vial before opening. Dilute to the working concentration using Biotin-antibody Diluent(1:100), respectively.
4. HRP-avidin Centrifuge the vial before opening. Dilute to the working concentration using HRP-avidin Diluent(1:100), respectively.
Precaution: The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
OTHER SUPPLIES REQUIRED
Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
Pipettes and pipette tips.
Deionized or distilled water.
Squirt bottle, manifold dispenser, or automated microplate washer.
An incubator which can provide stable incubation conditions up to 37°C±0.5°C.
SAMPLE COLLECTION AND STORAGE
l Serum Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at 1000 g. Remove serum and assay immediately or aliquot and store samples at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
l Plasma Collect plasma using citrate, EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 g within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
l Tissue Homogenates 100mg tissue was rinsed with 1X PBS, homogenized in 1 mL of 1X PBS and stored overnight at -20° C. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C. The supernate was assayed and removed immediately. Alternatively, aliquot and store samples at -20°C or -80℃. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
Note: Grossly hemolyzed samples are not suitable for use in this assay.
ASSAY PROCEDURE
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate. All the reagents should be added directly to the liquid level in the well. The pipette should avoid contacting the inner wall of the well.
1. Add 100μl of Standard, Blank, or Sample per well. Cover with the adhesive strip. Incubate for 2 hours at 37°C.
2. Remove the liquid of each well, don’t wash.
3. Add 100μl of Biotin-antibody working solution to each well. Incubate for 1 hour at 37°C. Biotin-antibody working solution may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.
4. Aspirate each well and wash, repeating the process three times for a total of three washes. Wash: Fill each well with Wash Buffer (200μl) and let it stand for 2 minutes, then remove the liquid by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel. Complete removal of liquid at each step is essential to good performance.
5. Add 100μl of HRP-avidin working solution to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
6. Repeat the aspiration and wash five times as step 4.
7. Add 90μl of TMB Substrate to each well. Incubate for 10-30 minutes at 37°C. Keeping the plate away from drafts and other temperature fluctuations in the dark.
8. Add 50μl of Stop Solution to each well when the first four wells containing the highest concentration of standards develop obvious blue color. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.
9. Determine the optical density of each well within 30 minutes, using a microplate reader set to 450 nm.
CALCULATION OF RESULTS
Using the professional soft "Curve Exert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard, control, and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL-10 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
LIMITATIONS OF THE PROCEDURE
The kit should not be used beyond the expiration date on the kit label.
Do not mix or substitute reagents with those from other lots or sources.
It is important that the Standard Diluent selected for the standard curve be consistent with the samples being assayed.
If samples generate values higher than the highest standard, dilute the samples with the appropriate Standard Diluent and repeat the assay.
Any variation in Standard Diluent, operator, pipetting technique, washing technique, incubation time or temperature, and kit age can cause variation in binding.
This assay is designed to eliminate interference by soluble receptors, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
TECHNICAL HINTS
Centrifuge vials before opening to collect contents.
When mixing or reconstituting protein solutions, always avoid foaming.
To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.
When using an automated plate washer, adding a 30 second soak period following the addition of wash buffer, and/or rotating the plate 180 degrees between wash steps may improve assay precision.
To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary.
Substrate Solution should remain colorless or light blue until added to the plate. Keep Substrate Solution protected from light. Substrate Solution should change from colorless or light blue to gradations of blue.
Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution. Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.
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RJ518050旋轉(zhuǎn)關(guān)節(jié)產(chǎn)品特征:
Specifications | |||
Interface Type | 2.92 Female ( 50 ohms ) | Style | I Type |
Frequency Range | DC to 40 GHz | Phase WOW (Degrees Max.) | 1° |
VSWR, Max. | 1.30 @ DC to 10 GHz | Insertion Loss, ,Max. | 0.25 dB @ DC to 10 GHz |
1.45 @ 10 to 20 GHz | 0.40 dB @ 10 to 20 GHz | ||
1.60 @ 20 to 30 GHz | 0.55 dB @ 20 to 30 GHz | ||
1.75 @ 30 to 40 GHz | 0.70 dB @ 30 to 40 GHz | ||
VSWR WOW | 0.1 | Insertion Loss WOW | 0.02 dB |
Peak Power, Max. | 500 W | Average Power, Max. | 60 W @ 1 GHz |
Rotating Speed, Max. | 250 rpm | Life Time, Min. | 5 Million Revolutions |
Starting Torque | 3.5 Ncm. | Continuous Rotational Torque | 3.5 Ncm Max. |
Axial Load On Interface, Max | ± 1 N | Radial Load On Interface, Max. | ± 1 N |
Body Material | Stainless Steel | Contact Pin Material | Beryllium Copper |
DC Power Ability | 3 A * 24V @ Full RF Power | Contact Surface Finish | Gold Plate |
Weight | 0.014 kg | Marking | Adhesive Label |
Insulator Material | PTFE / Teflon | IP Protection Level | IP 64 Per EN 60529 |
Temperature(Ambient Range) | -40 to +60 °C (Operation) | Humidity | 85% (Operation) |
-50 to +70 °C (Storage) | (Non-Condensing), Max. | 95% (Storage) |
KEM自動(dòng)滴定儀AT-710系列KEM自動(dòng)滴定儀AT-710系列主要特點(diǎn):AT-710M:觸摸屏和操作單元之間無線連接,同時(shí)控制四臺(tái)自動(dòng)滴定測(cè)量單元。通過使用無線適配器(藍(lán)牙),操作單元和滴定裝置可以不用電纜連接。在測(cè)量樣品過程中有危害性氣體時(shí),可將滴定裝置放置在排煙柜中,由無線傳輸?shù)牟僮鲉卧獔?zhí)行滴定。AT-710M/AT-710S:滴定劑的信息存儲(chǔ)在滴定管上的芯片中。即使滴定管單元轉(zhuǎn)移到另一個(gè)滴定裝置,也不需要重新輸入滴定劑信息。AT-710M/AT-710S/AT-710B:滴定管單元特殊的開關(guān)閥門。減少滴定管和開關(guān)閥門間的死體積。特殊結(jié)構(gòu)的設(shè)計(jì)可減少滴定劑的使用量和快速更換滴定單元。AT-710M/AT-710S/AT-710B:智能化電極電纜。電極的信息可透過電纜上傳感器存儲(chǔ)相關(guān)資料。電極校準(zhǔn)的數(shù)據(jù)可應(yīng)用到不同的自動(dòng)滴定儀上。KEM自動(dòng)滴定儀AT-710系列技術(shù)參數(shù):
名稱 | 自動(dòng)電位滴定儀 | ||
儀器型號(hào) | AT-710M | AT-710S | AT-710B |
儀器組成 | MCU-710M+AT-710+螺旋槳或磁力攪拌器 | MCU-710S+AT-710+螺旋槳或磁力攪拌器 | AT-710+螺旋槳或磁力攪拌器 |
測(cè)量范圍 | 1)電位: -2000.0~2000.0mV2) pH: -20.000~20.000pH3)溫度: 0~100°C | ||
滴定方式 | 自動(dòng)滴定,自動(dòng)間歇滴定,間歇滴定,恒pH滴定 | ||
石油中和值滴定,COD滴定 | |||
滴定類型 | 電位滴定(酸堿,氧化還原,沉淀),光度滴定,極化滴定,電導(dǎo)滴定 | ||
終點(diǎn)判斷 | 全量滴定(自動(dòng)終點(diǎn)),自動(dòng)終點(diǎn)滴定,設(shè)定終點(diǎn)滴定 | ||
交叉點(diǎn)滴定,自動(dòng)終點(diǎn)/設(shè)定終點(diǎn)滴定 | |||
特殊應(yīng)用 | 量電極電位(pH, mV),酸解離常數(shù)(pKa) | ||
同時(shí)記錄雙通道電位,學(xué)習(xí)滴定 | |||
輸入設(shè)置 | 觸摸屏輸入 | 按鍵輸入 | |
顯示 | 1) 8.4英寸彩色液晶屏,800x600點(diǎn) | 1) LED背景光源液晶屏 | |
2)英文/日文/中文/韓文/俄文/西語(yǔ)/德語(yǔ)/法語(yǔ) | 2)英文/日文/中文/韓文/俄文/西語(yǔ) | ||
3)四個(gè)通道同時(shí)顯示 | 3)一個(gè)通道顯示 | 3)一個(gè)通道顯示 | |
計(jì)算 | 濃度計(jì)算,統(tǒng)計(jì)計(jì)算(平均值,標(biāo)準(zhǔn)差,相對(duì)標(biāo)準(zhǔn)差),自動(dòng)輸入空白和滴定度 | ||
數(shù)據(jù)儲(chǔ)存 | 500組樣品結(jié)果 | 50組樣品結(jié)果 | |
GLP認(rèn)證 | 登記操作者/使用群組管理滴定劑:提示滴定度測(cè)量日期/指示滴定劑剩余量/提示滴定管活塞更換日期/提示滴定劑換日期/滴定度測(cè)量履歷性能檢查:提醒計(jì)劃?rùn)z查日期/記錄檢查結(jié)果電極管理:記錄校正日期/記錄校正履歷/電極檢查/電極檢查履歷滴定管驗(yàn)證:驗(yàn)證/記錄驗(yàn)證結(jié)果時(shí)間管理:顯示操作時(shí)間 | 登記操作者/記錄檢查結(jié)果/電極校正記錄/滴定管精度確認(rèn)/時(shí)間管理 | |
滴定管單元 | 20mL玻璃滴定管附褐色保護(hù)套(標(biāo)配),選配: 10mL,5mL或1mL | ||
滴定管精確度 | 50mL滴定管(自動(dòng)注入器): ±0.5mL20mL滴定管: 0.02mL 重復(fù)性: 0.01mL10mL滴定管: 0.015mL 重復(fù)性: 0.005mL5mL滴定管: 0.01mL 重復(fù)性: 0.003mL1mL滴定管: 0.005mL 重復(fù)性: 0.001mL | ||
擴(kuò)大器 | 1) STD: pH(mV), mV,雙通道(標(biāo)配)2) PTA: pH(mV), mV,光度,三通道3) POA: pH(mV), mV,極化,三通道4) CMT: pH(mV), mV,電導(dǎo),三通道5) TET: pH(mV),pH(mv), mV,三通道 | ||
外部輸出 | RS-232C x3 | RS-232C x 2 | |
打印機(jī),電子天平,數(shù)據(jù)軟件(SOFT-CAP) | |||
USB x; 1 | USB x 1 | ||
U盤,熱敏打印機(jī),A4打印機(jī),鍵盤, 條碼機(jī),腳踏開關(guān), USB集線器 | U盤,熱敏打印機(jī),鍵盤,條碼機(jī),腳踏開關(guān), USB集線器,安卓設(shè)備 | ||
LAN x 1 :電腦(PC) | |||
擴(kuò)充功能 | 測(cè)量單元:電位滴定儀(AT-710)容量水分儀(MKV-710)庫(kù)侖水分儀MKC-710)最多四臺(tái)測(cè)量單元 | ||
自動(dòng)活塞滴定管:最多可控制10臺(tái)滴定管驅(qū)動(dòng)單元(包括主機(jī)兩臺(tái)) | |||
多樣品自動(dòng)進(jìn)樣器: CHA-600,CHA-700 | CHA-700 | ||
使用環(huán)境 | 1)溫度: 5~35°C2)相對(duì)濕度: 85%RH以下 | ||
電源 | AC100~240V,50Hz/60Hz | ||
耗電量 | 主機(jī):約30瓦打印機(jī):約7瓦 | 主機(jī):約20瓦打印機(jī):約7瓦 | |
尺寸 | 觸摸屏: 225(W) x 190(D) x 42(H)mm | ||
滴定單元: 141(W) x 296(D) x 367(H)mm(不包括管路)打印機(jī): 106(W) x 180(D) x 88(H)mm | |||
重量 | 觸摸屏:約1.5公斤 | ||
滴定單元:約4.0公斤打印機(jī):約0.4公斤 |
BQS2KW排污排沙潛水泵
產(chǎn)品介紹 BQS(W)礦用隔爆型排污排沙潛水電泵,是泵和電動(dòng)機(jī)組合一體的電力排灌設(shè)備,外型美觀,結(jié)構(gòu)簡(jiǎn)單,密封性能好,性能穩(wěn)定,壽命長(zhǎng),安裝使用方便,且可以長(zhǎng)期在水下連續(xù)作業(yè),被廣泛用于煤礦立井、斜井及井底散煤泥地自動(dòng)化排水。內(nèi)裝式電泵,水由電動(dòng)機(jī)夾層中流過,有效的延長(zhǎng)了電泵的使用壽命;外裝式電泵,葉輪采用雙流道大過流結(jié)構(gòu),運(yùn)行平穩(wěn),排污能力強(qiáng),防纏繞,無堵塞現(xiàn)象,并且有體積小,重量輕,攜帶方便的特點(diǎn)。結(jié)構(gòu)特點(diǎn) BQS(W)系列的礦用隔爆型排污排沙潛水電泵嚴(yán)格根據(jù)煤炭行業(yè)標(biāo)準(zhǔn)MT/T671-2005《煤礦用隔爆型潛水電泵》、中華人名共和國(guó)國(guó)家執(zhí)行標(biāo)準(zhǔn)GB3836-2000《爆炸性氣體環(huán)境用電氣設(shè)備》設(shè)計(jì)制作,電動(dòng)機(jī)采用Y型干式隔爆型三相異步電動(dòng)機(jī),具有啟動(dòng)轉(zhuǎn)矩大、運(yùn)行性能好、噪聲低、體積小、重量輕、維護(hù)方便等特點(diǎn),以及優(yōu)良的隔爆結(jié)構(gòu)性能和較高的防護(hù)等級(jí),適用于含有甲烷或煤塵等爆炸性氣體環(huán)境、有爆炸危險(xiǎn)的礦井下采掘面及巷道等場(chǎng)所與水泵配套使用。電泵采用O形橡膠密封圈作為靜密封,結(jié)構(gòu)性能好,采用橡膠硫化曲線控制技術(shù)保障了O型圈耐潮防老化的質(zhì)量及密封性能。動(dòng)密封采用兩套機(jī)械密封,并有油室隔離保護(hù),有效地為轉(zhuǎn)動(dòng)摩擦副提供潤(rùn)滑,形成流體膜潤(rùn)滑保護(hù),同時(shí)釋放在運(yùn)動(dòng)過程中積聚的熱量,從而延長(zhǎng)了機(jī)械密封的使用壽命,確保了密封性能。電泵的流體部件材質(zhì)采用耐磨合金鑄鐵,水泵部分采用流體機(jī)械設(shè)計(jì)及平衡技術(shù),有效的延長(zhǎng)了電泵的使用壽命。用途含有甲烷或煤塵爆炸危險(xiǎn)的煤礦井下采掘工作面排水,煤礦立井、斜井施工排水,礦井無水倉(cāng)井底主排水,主井井底散煤倉(cāng)自動(dòng)排水,老塘、無人區(qū)涌水自動(dòng)排水,煤礦井下含有煤塵、泥沙等較小固體顆粒的地下水排放,清水倉(cāng)排水,救災(zāi)搶險(xiǎn)排水,城市環(huán)境系統(tǒng)中污水排放處理使用條件輸送介質(zhì)溫度應(yīng)不超過40℃,含固體顆粒的介質(zhì)、其體積濃度一般不超過2%,輸送介質(zhì)pH值在4~10范圍內(nèi),工作環(huán)境溫度0~40℃,水泵潛入水深值不超過5m,介質(zhì)中固體最大粒直徑不大于水泵流道過流斷面最小尺寸的50%基本參數(shù)
型號(hào) | 流量 m3/h | 楊程 m | 功率 kW | 電壓 V | 電流 A | 額定 轉(zhuǎn)速 r/min | 電泵 效率 % | 通過顆粒最大直徑 mm |
15-15-2.2 | 15 | 15 | 2.2 | 380或660或1140 | 5.12或2.98 | 3000 | 33.3 | 10 |
25-10-2.2 | 25 | 10 | 34.5 |
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CKB型(B200型)單向楔塊超越離合器
CKB無內(nèi)環(huán)的單向楔塊型超越離合器基本參數(shù)和主要尺寸 長(zhǎng)度單位:mm
安裝參考范例(Mounting e×ample)
KDLU渦街流量計(jì)由儀華測(cè)控生產(chǎn),質(zhì)量可靠,售后有保障。聯(lián)系人:吳先生;聯(lián)系電話:傳真,QQ908805187本文來源<p><strong><a >壓力變送器</a></strong></p>,http://www.yhck88.com/,<p><strong><a >壓力變送器,<p><strong><a >壓力變送器</a></strong></p>產(chǎn)品名稱:KDLU渦街流量計(jì)產(chǎn)品價(jià)格:根據(jù)型號(hào)的不同而異型號(hào)規(guī)格:可根據(jù)用戶非標(biāo)定制產(chǎn)品類別:流量計(jì)公司簡(jiǎn)介:儀華測(cè)控儀表有限公司主要生產(chǎn)各種型號(hào)的壓力變送器、壓力傳感器、物位計(jì)、料位計(jì)、磁翻板物位計(jì)、溫度儀表、壓力儀表、物位儀表、控制開關(guān)、電熱帶配件等產(chǎn)品。是歷史三十多年的儀表儀器行業(yè)中的大型企業(yè),被國(guó)家列入大型一檔企業(yè),并被例入中國(guó)電力企業(yè)家協(xié)會(huì)火電分會(huì)成員,化工產(chǎn)業(yè)定點(diǎn)企業(yè)。被化工部、機(jī)電部、冶金工業(yè)部推薦為質(zhì)量信得過產(chǎn)品企業(yè)。由于儀華的誠(chéng)信和努力,長(zhǎng)期來在供求雙方之間保持著很高的信譽(yù)和良好的溝通渠道。企業(yè)連續(xù)多年被有關(guān)部門評(píng)為“重合同,守信用”單位。產(chǎn)品說明渦街流量計(jì)是綜合吸收發(fā)達(dá)國(guó)家先進(jìn)技術(shù)和總結(jié)多年研究生產(chǎn)經(jīng)驗(yàn)的基礎(chǔ)上進(jìn)行精心設(shè)計(jì)的產(chǎn)品,實(shí)現(xiàn)了產(chǎn)品智能化、標(biāo)準(zhǔn)化、系列化、通用化、生產(chǎn)模具化、確保產(chǎn)品質(zhì)量的美觀性。該產(chǎn)品具有電路先進(jìn)、功耗微低、量程比寬、結(jié)構(gòu)簡(jiǎn)單、阻力損失小、堅(jiān)固耐用、用途廣、使用壽命長(zhǎng)、工作穩(wěn)定、便于安裝調(diào)試等特點(diǎn)。1.無可動(dòng)部件,運(yùn)行可靠,性能較好,使用壽命長(zhǎng)。 2.測(cè)量被測(cè)流體,不直接接觸傳感器,性能穩(wěn)定。 3.輸出信號(hào)是與流量成正比的脈沖信號(hào)或輸出4~20mA標(biāo)準(zhǔn)電流信號(hào)。 5.測(cè)量量程比大,可達(dá)1:10。而差壓式只有1:3。 6.結(jié)構(gòu)簡(jiǎn)單而牢固,安裝方便,維修費(fèi)用極少。 KDLU渦街流量計(jì)工作原理: 在測(cè)量管中垂直插入一個(gè)柱狀物時(shí),流體通過柱狀物兩側(cè)就交替地產(chǎn)生有規(guī)則的旋渦(如圖所示),這種旋渦列被稱為卡門渦街。卡門渦街的釋放頻率與流體的流動(dòng)速度及柱狀物的寬度有關(guān),可用下式表示: f =St·v/d f-卡門渦街的釋放頻率 式中 :St-系數(shù)(稱為斯特羅哈數(shù)) v-流速 d-柱狀物的寬度 KDLU渦街流量計(jì)參數(shù)及要求1、測(cè)量介質(zhì): 氣體、液體、蒸氣 2、連接方式:法蘭卡裝式、法蘭式、插入式 3、口徑規(guī)格: 法蘭卡裝式口徑選擇 25,32,50,80,10 4、法蘭連接式口徑選擇 :100,150,200 KDLU渦街流量計(jì)流量測(cè)量范圍 :正常測(cè)量流速范圍(雷諾數(shù)1.5×104~4×106;氣體5~50m/s; 液體0.5~7m/s) 測(cè)量精度:1.0級(jí) 1.5級(jí) 被測(cè)介質(zhì)溫度:常溫–25℃~100℃,高溫–25℃~150℃ -25℃~250℃ KDLU渦街流量計(jì)產(chǎn)品工作原理: 在流體中設(shè)置三角柱型旋渦發(fā)生體,則從旋渦發(fā)生體兩側(cè)交替地產(chǎn)生有規(guī)則的旋渦,這種旋渦稱為卡門旋渦,如右圖所示,旋渦列在旋渦發(fā)生體下游非對(duì)稱地排列。 渦街流量計(jì)是根據(jù)卡門(Karman)渦街原理測(cè)量氣體、蒸汽或液體的體積流量、標(biāo)況的體積流量或質(zhì)量流量的體積流量計(jì)。并可作為流量變送器應(yīng)用于自動(dòng)化控制系統(tǒng)中。 渦街流量計(jì)是應(yīng)用流體振蕩原理來測(cè)量流量的,流體在管道中經(jīng)過渦街流量變送器時(shí),在三角柱的旋渦發(fā)生體后上下交替產(chǎn)生正比于流速的兩列旋渦,旋渦的釋放頻率與流過旋渦發(fā)生體的流體平均速度及旋渦發(fā)生體特征寬度有關(guān),可用下式表示:f=Stv/d 式中:f為旋渦的釋放頻率,Hz;v為流過旋渦發(fā)生體的流體平均速度,m/s;d為旋渦發(fā)生體特征寬度,m;St為斯特羅哈數(shù),無量綱,它的數(shù)值范圍為0.14-0.27。t是雷諾數(shù)的函數(shù),St=f(l/Re)。當(dāng)雷諾數(shù)Re在102~105范圍內(nèi),St值約為0.2,因此,在測(cè)量中,要盡量滿足流體的雷諾數(shù)在102~105,旋渦頻率f=0.2v/d。由此可知,通過測(cè)量旋渦頻率就可以計(jì)算出流過旋渦發(fā)生體的流體平均速度v,再由式q=vA可以求出流量q,其中A為流體流過旋渦發(fā)生體的截面積。功能1、表體中同時(shí)集成溫壓補(bǔ)償補(bǔ)償功能,可測(cè)量流體的標(biāo)準(zhǔn)體積流量或標(biāo)準(zhǔn)質(zhì)量流量2、全智能化、數(shù)字化電路設(shè)計(jì),可自動(dòng)補(bǔ)償被測(cè)流體密度或標(biāo)況體積計(jì)算。 3、 全新的數(shù)字濾波和修正功能使流量測(cè)量更加精準(zhǔn)可靠。 4、 電池供電型無需外接電源既可連續(xù)工作兩年以上。 5、 全新點(diǎn)陣漢字液晶顯示,使用操作更方便。
NKJ41真空隔離截止閥
產(chǎn)品用途
真空隔離截止閥適用于公稱壓力PN4.0~10.0Mpa,工作溫度≤200℃的火力電站回水冷凝系統(tǒng)管道上,切斷或接通中的介質(zhì)。適用介質(zhì)為:水、蒸汽等。
產(chǎn)品結(jié)構(gòu)特點(diǎn)
1.產(chǎn)品按JB/T3595標(biāo)準(zhǔn)設(shè)計(jì)制造,滿足電站設(shè)備配套使用要求。
2.設(shè)計(jì)了真空密封結(jié)構(gòu)填料室,隔離真空。
3.閥桿經(jīng)調(diào)質(zhì)及表面氮化處理,有良好的抗腐蝕性,抗擦傷和耐磨性。
4.法蘭墊片采用復(fù)合材料,膨脹系數(shù)大,氣密性能好,系統(tǒng)與大氣隔離。
5.倒密封采用不銹鋼螺紋連接密封座或本體堆焊奧氏體不銹鋼而成,倒密封。填料更換和維修可在不停機(jī)情況下進(jìn)行,方便快捷不影響系統(tǒng)運(yùn)行。
主要零件的材料說明
閥 體 | 25、WCB | Cr5Mo |
閥 蓋 | 25、WCB | Cr5Mo |
支 架 | 25、WCB | Cr5Mo |
閥 瓣 | 2Cr13、WCB | Cr5Mo |
閥 桿 | 2Cr13 | 38CrMoAL、25Cr2MoV |
閥 座 | 25、304 | 304 |
密封環(huán) | 膨脹柔性石墨 | |
填 料 | 膨脹柔性石墨 | |
閥桿螺母 | ZCuA110Fe3 |
技術(shù)參數(shù):
主版尺寸 | 4.6cm×7.1cm×2.8cm |
重量 | 64g(主板) |
輸入電壓 | +8-18VDC 或+18-28VDC(用戶可選) |
電流 | 最大40mA |
鋁外殼封裝 | 8.0cm × 7.5cm × 5.7cm |
重量 | 400g(整機(jī)) |
精度 | ±0.5°(±10mils) |
可重復(fù)性 | ±0.2°(±5mils) |
分辨率 | ±0.1°(1mil) |
傾斜 | ±16°(SE-25) ±45°(SE-10不帶殼) |
響應(yīng)時(shí)間 | 0.1 -24秒可調(diào) |
操作溫度 | -40℃-+65℃ |
存貯溫度 | -51℃-+71℃ |
沖擊/振動(dòng) | 符合美MIL-STD-810 |
標(biāo)準(zhǔn)平均無故障時(shí)間 | 大于30000小時(shí) |